PrimePCR™ Probe Assay: IFRD2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: IFRD2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: IFRD2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058598
Assay Design:   exonic
Chromosome Location:   3:50325847-50326014question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000361125 ENST00000361368 ENST00000568627 ENST00000249016 ENST00000296597 ENST00000511107 ENST00000502658 ENST00000075503 ENST00000417626 ENST00000426499 ENST00000436390 ENST00000336089 ENST00000429673 ENST00000349769 ENST00000545908 ENST00000216605 ENST00000555709 ENST00000367265 ENST00000367264 ENST00000235790 ENST00000575887 ENST00000576857 ENST00000571492

Gene Information

Description Not Available

Gene Symbol:   IFRD2
Gene Name:   interferon-related developmental regulator 2
Aliases:   FLJ40446, IFNRP, SKMc15, SM15
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000214706
Entrez:   7866
Chromosome Mapping:   3p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.390000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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