PrimePCR™ Probe Assay: DPF2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best efficiency

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: DPF2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DPF2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058546
Assay Design:   exonic
Chromosome Location:   11:65119228-65119337question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000528416 ENST00000415073 ENST00000252268 ENST00000531989

Gene Information

The protein encoded by this gene is a member of the d4 domain family characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It likely serves a regulatory role in rapid hematopoietic cell growth and turnover. This gene is considered a candidate gene for multiple endocrine neoplasia type I an inherited cancer syndrome involving multiple parathyroid enteropancreatic and pituitary tumors. [provided by RefSeq Jul 2008]

Gene Symbol:   DPF2
Gene Name:   D4, zinc and double PHD fingers family 2
Aliases:   MGC10180, REQ, UBID4, ubi-d4
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000133884
Entrez:   5977
Chromosome Mapping:   11q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.700000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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