PrimePCR™ Probe Assay: CAMKV, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058440
Assay Design:   exonic
Chromosome Location:   3:49898150-49898261question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000296471 ENST00000488336 ENST00000463537 ENST00000477224 ENST00000467248 ENST00000466940

Gene Information

Description Not Available

Gene Symbol:   CAMKV
Gene Name:   CaM kinase-like vesicle-associated
Aliases:   1G5, MGC8407, VACAMKL
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000164076
Entrez:   79012
Chromosome Mapping:   3p21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.410000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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