PrimePCR™ Probe Assay: RHOF, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RHOF, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RHOF, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058423
Assay Design:   exonic
Chromosome Location:   12:122231143-122231501question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000267205 ENST00000535560 ENST00000286301 ENST00000379442 ENST00000536621 ENST00000370401 ENST00000432680 ENST00000262858 ENST00000426613 ENST00000284548 ENST00000570156 ENST00000441106 ENST00000422127 ENST00000366707 ENST00000366709 ENST00000599144 ENST00000596765 ENST00000600022 ENST00000593922 ENST00000599223 ENST00000597198 ENST00000377139 ENST00000601291 ENST00000596822 ENST00000600911 ENST00000309877 ENST00000349945 ENST00000432919 ENST00000566899 ENST00000393742 ENST00000567239 ENST

Gene Information

Description Not Available

Gene Symbol:   RHOF
Gene Name:   ras homolog gene family, member F (in filopodia)
Aliases:   ARHF, FLJ20247, RIF
RefSeq:   NC_000012.11 NT_009775.17
Ensembl:   ENSG00000139725
Entrez:   54509
Chromosome Mapping:   12q24.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.310000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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