This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: LIFR, Human
PrimePCR™ Template for Probe Assay: LIFR, Human
This gene encodes a protein that belongs to the type I cytokine receptor family. This protein combines with a high-affinity converter subunit gp130 to form a receptor complex that mediates the action of the leukemia inhibitory factor a polyfunctional cytokine that is involved in cellular differentiation proliferation and survival in the adult and the embryo. Mutations in this gene cause Schwartz-Jampel syndrome type 2 a disease belonging to the group of the bent-bone dysplasias. A translocation that involves the promoter of this gene t(5;8)(p13;q12) with the pleiomorphic adenoma gene 1 is associated with salivary gland pleiomorphic adenoma a common type of benign epithelial tumor of the salivary gland. Multiple splice variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.