PrimePCR™ Probe Assay: LIFR, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058374
Assay Design:   exonic
Chromosome Location:   5:38481779-38481884question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000263409 ENST00000453190

Gene Information

This gene encodes a protein that belongs to the type I cytokine receptor family. This protein combines with a high-affinity converter subunit gp130 to form a receptor complex that mediates the action of the leukemia inhibitory factor a polyfunctional cytokine that is involved in cellular differentiation proliferation and survival in the adult and the embryo. Mutations in this gene cause Schwartz-Jampel syndrome type 2 a disease belonging to the group of the bent-bone dysplasias. A translocation that involves the promoter of this gene t(5;8)(p13;q12) with the pleiomorphic adenoma gene 1 is associated with salivary gland pleiomorphic adenoma a common type of benign epithelial tumor of the salivary gland. Multiple splice variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   LIFR
Gene Name:   leukemia inhibitory factor receptor alpha
Aliases:   CD118, FLJ98106, FLJ99923, LIF-R, SJS2, STWS, SWS
RefSeq:   NC_000005.9 NT_006576.16 NG_011817.1
Ensembl:   ENSG00000113594
Entrez:   3977
UniGene:   Hs.133421
Chromosome Mapping:   5p13-p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 35.970000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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