PrimePCR™ Probe Assay: PSG6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PSG6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PSG6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058356
Assay Design:   exonic
Chromosome Location:   19:43420502-43421882question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000327495 ENST00000595140 ENST00000404209 ENST00000401467 ENST00000306511 ENST00000436291 ENST00000595124 ENST00000595356 ENST00000403380 ENST00000312439 ENST00000244296 ENST00000595930 ENST00000187910 ENST00000402603 ENST00000292125 ENST00000601833 ENST00000320078 ENST00000598133 ENST00000401740 ENST00000406487 ENST00000405312 ENST00000244295 ENST00000599746 ENST00000451895 ENST00000600572 ENST00000433626

Gene Information

The human pregnancy-specific glycoproteins (PSGs) are a group of molecules that are mainly produced by the placental syncytiotrophoblasts during pregnancy. PSGs comprise a subgroup of the carcinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily. For additional general information about the PSG gene family see PSG1 (MIM 176390).[supplied by OMIM Oct 2009]

Gene Symbol:   PSG6
Gene Name:   pregnancy specific beta-1-glycoprotein 6
Aliases:   PSBG-10, PSBG-12, PSBG-6, PSG10
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000170848
Entrez:   5675
Chromosome Mapping:   19q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.890000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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