PrimePCR™ Probe Assay: SCN2A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058345
Assay Design:   exonic
Chromosome Location:   2:166166953-166167078question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000424833 ENST00000283256 ENST00000375427 ENST00000375437 ENST00000357398

Gene Information

Voltage-gated sodium channels are transmembrane glycoprotein complexes composed of a large alpha subunit with 24 transmembrane domains and one or more regulatory beta subunits. They are responsible for the generation and propagation of action potentials in neurons and muscle. This gene encodes one member of the sodium channel alpha subunit gene family. It is heterogeneously expressed in the brain and mutations in this gene have been linked to several seizure disorders. Several alternatively spliced transcript variants of this gene have been described but the full-length nature of some of these variants has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   SCN2A
Gene Name:   sodium channel, voltage-gated, type II, alpha subunit
Aliases:   BFIC3, EIEE11, HBA, HBSCI, HBSCII, NAC2, Na(v)1.2, Nav1.2, SCN2A1, SCN2A2
RefSeq:   NC_000002.11 NG_008143.1 NT_005403.17
Ensembl:   ENSG00000136531
Entrez:   6326
Chromosome Mapping:   2q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.600000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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