PrimePCR™ Probe Assay: DGKZ, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: DGKZ, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DGKZ, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058242
Assay Design:   exonic
Chromosome Location:   11:46396504-46396983question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000436407 ENST00000589891 ENST00000530004 ENST00000447632 ENST00000413473 ENST00000587757 ENST00000399847 ENST00000343674 ENST00000528615 ENST00000532868 ENST00000527911 ENST00000456247 ENST00000421244 ENST00000454345 ENST00000524869 ENST00000395574 ENST00000318201 ENST00000303004 ENST00000554329 ENST00000556585 ENST00000418511 ENST00000531445 ENST00000576988 ENST00000576570 ENST00000573759 ENST00000381165 ENST00000357681 ENST00000341360 ENST00000367218 ENST00000367219 ENST00000391954 ENST

Gene Information

The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and multiple transcript variants encoding distinct isoforms have been identified. [provided by RefSeq Nov 2010]

Gene Symbol:   DGKZ
Gene Name:   diacylglycerol kinase, zeta
Aliases:   DAGK5, DAGK6, DGK-ZETA, hDGKzeta
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000149091
Entrez:   8525
Chromosome Mapping:   11p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 35.970000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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