PrimePCR™ Probe Assay: C1GALT1C1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: C1GALT1C1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: C1GALT1C1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058194
Assay Design:   exonic
Chromosome Location:   X:119760150-119760257question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000304661 ENST00000371313

Gene Information

This gene encodes a type II transmembrane protein that is similar to the core 1 beta13-galactosyltransferase 1 which catalyzes the synthesis of the core-1 structure also known as Thomsen-Friedenreich antigen on O-linked glycans. This gene product lacks the galactosyltransferase activity itself but instead acts as a molecular chaperone required for the folding stability and full activity of the core 1 beta13-galactosyltransferase 1. Mutations in this gene have been associated with Tn syndrome. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq Dec 2009]

Gene Symbol:   C1GALT1C1
Gene Name:   C1GALT1-specific chaperone 1
Aliases:   C1GALT2, C38H2-L1, COSMC, MGC19947, MST143
RefSeq:   NC_000023.10 NT_011786.16 NG_016219.1
Ensembl:   ENSG00000171155
Entrez:   29071
Chromosome Mapping:   Xq24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.570000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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