PrimePCR™ Probe Assay: LCE3B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: LCE3B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LCE3B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058171
Assay Design:   exonic
Chromosome Location:   1:152586448-152586543question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000573484 ENST00000568193 ENST00000564962 ENST00000565557 ENST00000565080 ENST00000561589 ENST00000561919 ENST00000567302 ENST00000565268 ENST00000371070 ENST00000315150 ENST00000393304 ENST00000393308 ENST00000393307 ENST00000393305 ENST00000418265 ENST00000338820 ENST00000369209 ENST00000345136 ENST00000357649 ENST00000354589 ENST00000398774 ENST00000322810 ENST00000354958 ENST00000356346 ENST00000436759 ENST00000333881 ENST00000335633 ENST00000335674

Gene Information

Description Not Available

Gene Symbol:   LCE3B
Gene Name:   late cornified envelope 3B
Aliases:   LEP14
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000187238
Entrez:   353143
Chromosome Mapping:   1q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.970000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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