PrimePCR™ Probe Assay: KCNJ16, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058151
Assay Design:   exonic
Chromosome Location:   17:68127100-68128253question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000392670 ENST00000392671 ENST00000283936 ENST00000587892 ENST00000587698 ENST00000585558 ENST00000586462 ENST00000589377 ENST00000591891

Gene Information

Potassium channels are present in most mammalian cells where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein which has a greater tendency to allow potassium to flow into a cell rather than out of a cell can form heterodimers with two other inward-rectifier type potassium channels. It may be involved in the regulation of fluid and pH balance. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   KCNJ16
Gene Name:   potassium inwardly-rectifying channel, subfamily J, member 16
Aliases:   BIR9, KIR5.1, MGC33717
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000153822
Entrez:   3773
UniGene:   Hs.463985
Chromosome Mapping:   17q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.990000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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