PrimePCR™ Probe Assay: RGS3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057987
Assay Design:   exonic
Chromosome Location:   9:116359120-116359217question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000374140 ENST00000343817 ENST00000462143 ENST00000374134 ENST00000462403 ENST00000350696 ENST00000394646 ENST00000342620

Gene Information

This gene encodes a member of the regulator of G-protein signaling (RGS) family. This protein is a GTP-ase activating protein which inhibits G-protein mediated signal transduction. The protein is largely cytosolic but G-protein activation leads to translocation of this protein to the plasma membrane. A nuclear form of this protein has also been described but its sequence has not been identified. Multiple alternatively spliced transcript variants have been described for this gene but the full-length nature of some transcripts is not yet known. [provided by RefSeq Jul 2008]

Gene Symbol:   RGS3
Gene Name:   regulator of G-protein signaling 3
Aliases:   C2PA, FLJ20370, FLJ31516, FLJ90496, PDZ-RGS3, RGP3
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000138835
Entrez:   5998
UniGene:   Hs.494875
Chromosome Mapping:   9q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.780000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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