PrimePCR™ Probe Assay: SPAG11B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SPAG11B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SPAG11B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057975
Assay Design:   exonic
Chromosome Location:   8:7321065-7321176question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000389282 ENST00000403903 ENST00000315614 ENST00000399464 ENST00000570460 ENST00000571887 ENST00000400125 ENST00000434307 ENST00000545921 ENST00000379887 ENST00000297498 ENST00000317900 ENST00000361574 ENST00000587975 ENST00000340305 ENST00000278379 ENST00000395750 ENST00000395753 ENST00000366835 ENST00000590201 ENST00000589168 ENST00000589711 ENST00000225777

Gene Information

This gene encodes several androgen-dependent epididymis-specific secretory proteins. The specific functions of these proteins have not been determined but they are thought to be involved in sperm maturation. Some of the isoforms contain regions of similarity to beta-defensins a family of antimicrobial peptides. The gene is located on chromosome 8p23 near the defensin gene cluster. Alternative splicing of this gene results in seven transcript variants encoding different isoforms. Two different N-terminal and five different C-terminal protein sequences are encoded by the splice variants. Two additional variants have been described but their full length sequences have not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   SPAG11B
Gene Name:   sperm associated antigen 11B
Aliases:   EDDM2B, EP2, EP2C, EP2D, HE2, HE2C, MGC61846, SPAG11
RefSeq:   NC_000008.10 NT_023736.17
Ensembl:   ENSG00000164871
Entrez:   10407
Chromosome Mapping:   8p23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.560000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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