PrimePCR™ Probe Assay: POR, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: POR, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: POR, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057953
Assay Design:   exonic
Chromosome Location:   7:75615675-75615780question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000343933 ENST00000375077 ENST00000461988 ENST00000394893 ENST00000447222 ENST00000439269 ENST00000545601 ENST00000450476 ENST00000419840 ENST00000573555 ENST00000597908 ENST00000597647 ENST00000593343 ENST00000595553 ENST00000593927 ENST00000595759

Gene Information

This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors FAD and FMN which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases including apparent combined P450C17 and P450C21 deficiency amenorrhea and disordered steroidogenesis congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq Jul 2008]

Gene Symbol:   POR
Gene Name:   P450 (cytochrome) oxidoreductase
Aliases:   CPR, CYPOR, DKFZp686G04235, FLJ26468, P450R
RefSeq:   NC_000007.13 NG_008930.1 NT_007933.15
Ensembl:   ENSG00000127948
Entrez:   5447
Chromosome Mapping:   7q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.710000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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