PrimePCR™ Probe Assay: SLC25A22, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SLC25A22, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SLC25A22, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057942
Assay Design:   exonic
Chromosome Location:   11:791215-791444question
Amplicon Length:   200
Splice Variants Targeted:   ENST00000320230 ENST00000394458 ENST00000404085 ENST00000594072 ENST00000598347 ENST00000433424 ENST00000468090 ENST00000291565 ENST00000467908 ENST00000367080

Gene Information

This gene encodes a mitochondrial glutamate carrier. Mutations in this gene are associated with early infantile epileptic encephalopathy. Multiple alternatively spliced variants encoding the same protein have been identified.[provided by RefSeq Jul 2010]

Gene Symbol:   SLC25A22
Gene Name:   solute carrier family 25 (mitochondrial carrier: glutamate), member 22
Aliases:   EIEE3, FLJ13044, GC1, NET44
RefSeq:   NC_000011.9 NT_009237.18 NG_023407.1
Ensembl:   ENSG00000177542
Entrez:   79751
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.830000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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