PrimePCR™ Probe Assay: BSG, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: BSG, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: BSG, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057940
Assay Design:   exonic
Chromosome Location:   19:578121-579590question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000539295 ENST00000237275 ENST00000416313 ENST00000542548 ENST00000322475 ENST00000280057 ENST00000388715 ENST00000394571 ENST00000381580 ENST00000303415 ENST00000329410 ENST00000221347 ENST00000545507 ENST00000333511 ENST00000573216 ENST00000353555 ENST00000573784 ENST00000576984 ENST00000371218 ENST00000303721 ENST00000344733 ENST00000382788 ENST00000338806 ENST00000336727 ENST00000306648 ENST00000538395 ENST00000354376 ENST00000510568 ENST00000341156 ENST00000418555 ENST00000449184 ENST

Gene Information

The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis embryo implantation neural network formation and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   BSG
Gene Name:   basigin (Ok blood group)
Aliases:   5F7, CD147, EMMPRIN, M6, OK, TCSF
RefSeq:   NC_000019.9 NT_011255.14 NG_007468.1
Ensembl:   ENSG00000172270
Entrez:   682
Chromosome Mapping:   19p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.850000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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