PrimePCR™ Probe Assay: EPHA10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: EPHA10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EPHA10, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057905
Assay Design:   exonic
Chromosome Location:   1:38220041-38227247question
Amplicon Length:   143
Splice Variants Targeted:   ENST00000216044 ENST00000572888 ENST00000342782 ENST00000373048 ENST00000319637 ENST00000427468 ENST00000397360 ENST00000394729 ENST00000330452 ENST00000344204 ENST00000344135 ENST00000537187 ENST00000259808 ENST00000541791 ENST00000380409 ENST00000599661

Gene Information

Ephrin receptors the largest subfamily of receptor tyrosine kinases (RTKs) and their ephrin ligands are important mediators of cell-cell communication regulating cell attachment shape and mobility in neuronal and epithelial cells (Aasheim et al. 2005 [PubMed 15777695]). See MIM 179610 for additional background on Eph receptors and ephrins.[supplied by OMIM Mar 2008]

Gene Symbol:   EPHA10
Gene Name:   EPH receptor A10
Aliases:   FLJ16103, FLJ33655, MGC43817
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000183317
Entrez:   284656
Chromosome Mapping:   1p34.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.990000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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