PrimePCR™ Probe Assay: TBC1D3B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TBC1D3B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TBC1D3B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057899
Assay Design:   exonic
Chromosome Location:   17:34499315-34499758question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000574788 ENST00000250066 ENST00000332776 ENST00000304328 ENST00000454519 ENST00000586369 ENST00000589443 ENST00000398801 ENST00000336331 ENST00000457979 ENST00000591555 ENST00000308078 ENST00000451448 ENST00000394359 ENST00000455054 ENST00000535446 ENST00000400684 ENST00000394453 ENST00000592614 ENST00000341264 ENST00000591542 ENST00000592987 ENST00000330458 ENST00000535592 ENST00000535805 ENST00000327454 ENST00000505415 ENST00000378174 ENST00000539424 ENST00000518551 ENST00000354664 ENST

Gene Information

This gene encodes a protein that is similar to TBC1 domain family member 3. This protein contains a TBC (Tre-2 Bub2p and Cdc16p) domain which is found in proteins involved in RAB GTPase signaling and vesicle trafficking. There are multiple copies of this gene located within a cluster of chemokine genes on chromosome 17q. [provided by RefSeq Apr 2009]

Gene Symbol:   TBC1D3B
Gene Name:   TBC1 domain family, member 3B
Aliases:   FLJ78399, PRC17, TBC1D3I
RefSeq:   NC_000017.10 NG_004113.1 NT_010799.15 NW_003315949.1
Ensembl:   ENSG00000224226
Entrez:   414059
Chromosome Mapping:   17q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.680000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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