PrimePCR™ Probe Assay: BRF1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: BRF1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: BRF1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057889
Assay Design:   exonic
Chromosome Location:   14:105707626-105707750question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000392557 ENST00000379937 ENST00000546474 ENST00000551787 ENST00000327359 ENST00000440513 ENST00000549655 ENST00000552127 ENST00000546417 ENST00000550208 ENST00000550375 ENST00000379932 ENST00000380445 ENST00000380469

Gene Information

This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA 5S rRNA and other small structural RNAs. The gene product belongs to the TF2B family. Several alternatively spliced variants encoding different isoforms that function at different promoters transcribed by RNA polymerase III have been identified. [provided by RefSeq Jun 2011]

Gene Symbol:   BRF1
Gene Name:   BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae)
Aliases:   BRF, BRF-1, FLJ42674, FLJ43034, GTF3B, MGC105048, TAF3B2, TAF3C, TAFIII90, TF3B90, TFIIIB90, hBRF
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000185024
Entrez:   2972
Chromosome Mapping:   14q

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 40.010000
Efficiency 87

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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