PrimePCR™ Probe Assay: CPSF3L, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CPSF3L, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CPSF3L, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057879
Assay Design:   exonic
Chromosome Location:   1:1250215-1250304question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000435064 ENST00000411962 ENST00000421495 ENST00000419704 ENST00000450926 ENST00000434694 ENST00000526332 ENST00000540437 ENST00000545578

Gene Information

The Integrator complex contains at least 12 subunits and associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates the 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690). INTS11 or CPSF3L is the catalytic subunit of the Integrator complex (Baillat et al. 2005 [PubMed 16239144]).[supplied by OMIM Mar 2008]

Gene Symbol:   CPSF3L
Gene Name:   cleavage and polyadenylation specific factor 3-like
Aliases:   CPSF73L, FLJ13294, FLJ20542, INT11, INTS11, RC-68, RC68
RefSeq:   NC_000001.10 NT_004350.19
Ensembl:   ENSG00000127054
Entrez:   54973
Chromosome Mapping:   1p36.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.690000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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