PrimePCR™ Probe Assay: SH3GL1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SH3GL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SH3GL1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057855
Assay Design:   exonic
Chromosome Location:   19:4362720-4363730question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000586237 ENST00000342216 ENST00000242783 ENST00000252597 ENST00000431146 ENST00000392017 ENST00000373525 ENST00000392020 ENST00000392018 ENST00000347464 ENST00000417856 ENST00000261405 ENST00000528896 ENST00000389003 ENST00000544884 ENST00000376477 ENST00000515384 ENST00000269886 ENST00000598564 ENST00000417295 ENST00000330175 ENST00000285206 ENST00000503131 ENST00000270560 ENST00000585881 ENST00000600735

Gene Information

This gene encodes a member of the endophilin family of Src homology 3 domain-containing proteins. The encoded protein is involved in endocytosis and may also play a role in the cell cycle. Overexpression of this gene may play a role in leukemogenesis and the encoded protein has been implicated in acute myeloid leukemia as a fusion partner of the myeloid-lymphoid leukemia protein. Pseudogenes of this gene are located on the long arm of chromosomes 11 and 17. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq Jan 2011]

Gene Symbol:   SH3GL1
Gene Name:   SH3-domain GRB2-like 1
Aliases:   CNSA1, EEN, MGC111371, SH3D2B, SH3P8
RefSeq:   NC_000019.9 NG_028084.1 NT_011255.14
Ensembl:   ENSG00000141985
Entrez:   6455
Chromosome Mapping:   19p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 37.650000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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