PrimePCR™ Probe Assay: KIFC3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
Your Price:   Log In
Quantity:  
 

PrimePCR™ PreAmp for Probe Assay: KIFC3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: KIFC3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057852
Assay Design:   exonic
Chromosome Location:   16:57798159-57799490question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000409429 ENST00000233668 ENST00000340004 ENST00000264012 ENST00000429102 ENST00000581171 ENST00000455895 ENST00000341071 ENST00000413080 ENST00000257013 ENST00000264501 ENST00000438707 ENST00000306802 ENST00000388738 ENST00000369902 ENST00000369899 ENST00000423559 ENST00000465878 ENST00000379655 ENST00000445690 ENST00000421376 ENST00000541240 ENST00000540079 ENST00000543930 ENST00000562903 ENST00000539578 ENST00000413420 ENST00000600009 ENST00000593285 ENST00000599270 ENST00000597902 ENST

Gene Information

KIFC3 belongs to the large superfamily of kinesins molecular motors that use the energy of ATP hydrolysis to translocate cargoes along microtubules. Members share extensive homology within a globular domain containing the microtubule- and ATP-binding sites and have a coiled-coil stalk domain that mediates oligomerization. Different kinesin family members participate in specific and diverse motile processes such as cell division organelle transport and nuclear movement (Hoang et al. 1998 [PubMed 9782090]).[supplied by OMIM Mar 2009]

Gene Symbol:   KIFC3
Gene Name:   kinesin family member C3
Aliases:   DKFZp686D23201, FLJ34694
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000140859
Entrez:   3801
Chromosome Mapping:   16q13-q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.660000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download