PrimePCR™ Probe Assay: PLA2G6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PLA2G6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PLA2G6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057813
Assay Design:   exonic
Chromosome Location:   22:38507885-38508020question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000335539 ENST00000402064 ENST00000332509

Gene Information

The protein encoded by this gene is an A2 phospholipase a class of enzyme that catalyzes the release of fatty acids from phospholipids. The encoded protein may play a role in phospholipid remodelling arachidonic acid release leukotriene and prostaglandin synthesis fas-mediated apoptosis and transmembrane ion flux in glucose-stimulated B-cells. Several transcript variants encoding multiple isoforms have been described but the full-length nature of only three of them have been determined to date. [provided by RefSeq Dec 2010]

Gene Symbol:   PLA2G6
Gene Name:   phospholipase A2, group VI (cytosolic, calcium-independent)
Aliases:   CaI-PLA2, GVI, INAD1, IPLA2-VIA, NBIA2A, NBIA2B, PARK14, PLA2, PNPLA9, iPLA2
RefSeq:   NC_000022.10 NT_011520.12 NG_007094.1
Ensembl:   ENSG00000184381
Entrez:   8398
Chromosome Mapping:   22q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.800000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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