PrimePCR™ Probe Assay: SFTPC, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SFTPC, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SFTPC, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057801
Assay Design:   exonic
Chromosome Location:   8:22019377-22020181question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000318561 ENST00000521315 ENST00000437090 ENST00000522109 ENST00000518615

Gene Information

This gene encodes the pulmonary-associated surfactant protein C (SPC) an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA SPB SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. Multiple mutations in this gene have been identified which cause pulmonary surfactant metabolism dysfunction type 2 also called pulmonary alveolar proteinosis due to surfactant protein C deficiency and are associated with interstitial lung disease in older infants children and adults. Alternatively spliced transcript variants encoding different protein isoforms have been identified.[provided by RefSeq Feb 2010]

Gene Symbol:   SFTPC
Gene Name:   surfactant protein C
Aliases:   PSP-C, SFTP2, SMDP2, SP-C
RefSeq:   NC_000008.10 NT_167187.1 NG_016968.1
Ensembl:   ENSG00000168484
Entrez:   6440
Chromosome Mapping:   8p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.220000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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