PrimePCR™ Probe Assay: CADPS, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CADPS, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CADPS, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057796
Assay Design:   exonic
Chromosome Location:   3:62452070-62459945question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000383710 ENST00000473635 ENST00000357948 ENST00000283269 ENST00000466621

Gene Information

This gene encodes a novel neural/endocrine-specific cytosolic and peripheral membrane protein required for the Ca2+-regulated exocytosis of secretory vesicles. The protein acts at a stage in exocytosis that follows ATP-dependent priming which involves the essential synthesis of phosphatidylinositol 45-bisphosphate (PtdIns(45)P2). Alternative splicing has been observed at this locus and three variants encoding distinct isoforms are described. [provided by RefSeq Aug 2008]

Gene Symbol:   CADPS
Gene Name:   Ca++-dependent secretion activator
Aliases:   CADPS1, CAPS, CAPS1, KIAA1121
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000163618
Entrez:   8618
Chromosome Mapping:   3p14.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.370000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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