PrimePCR™ Probe Assay: PCBP4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PCBP4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PCBP4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057710
Assay Design:   exonic
Chromosome Location:   3:51991900-51992007question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000355852 ENST00000461554 ENST00000484633 ENST00000471622 ENST00000322099 ENST00000428823 ENST00000395014 ENST00000395013

Gene Information

This gene encodes a member of the KH-domain protein subfamily. Proteins of this subfamily also referred to as alpha-CPs bind to RNA with a specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. This gene is induced by the p53 tumor suppressor and the encoded protein can suppress cell proliferation by inducing apoptosis and cell cycle arrest in G(2)-M. This gene's protein is found in the cytoplasm yet it lacks the nuclear localization signals found in other subfamily members. Multiple alternatively spliced transcript variants have been described but the full-length nature for only some has been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   PCBP4
Gene Name:   poly(rC) binding protein 4
Aliases:   CBP, LIP4, MCG10
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000090097
Entrez:   57060
Chromosome Mapping:   3p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.300000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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