PrimePCR™ Probe Assay: PALLD, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057606
Assay Design:   exonic
Chromosome Location:   4:169847478-169847613question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000261509 ENST00000512127 ENST00000507735 ENST00000335742

Gene Information

This gene encodes a cytoskeletal protein that is required for organizing the actin cytoskeleton. The protein is a component of actin-containing microfilaments and it is involved in the control of cell shape adhesion and contraction. Polymorphisms in this gene are associated with a susceptibility to pancreatic cancer type 1 and also with a risk for myocardial infarction. Alternative splicing results in multiple transcript variants. [provided by RefSeq Oct 2009]

Gene Symbol:   PALLD
Gene Name:   palladin, cytoskeletal associated protein
Aliases:   CGI151, FLJ22190, FLJ38193, FLJ39139, FLJ61376, KIAA0992, MYN, PNCA1, SIH002
RefSeq:   NC_000004.11 NT_016354.19 NG_013376.1
Ensembl:   ENSG00000129116
Entrez:   23022
UniGene:   Hs.151220
Chromosome Mapping:   4q32.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.720000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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