PrimePCR™ Probe Assay: NDEL1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NDEL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NDEL1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057602
Assay Design:   exonic
Chromosome Location:   17:8350100-8350212question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000299734 ENST00000402554 ENST00000380025 ENST00000334527 ENST00000582665 ENST00000582812

Gene Information

This gene encodes a coiled-coil protein that plays a role in multiple processes including cytoskeletal organization cell signaling and neuron migration outgrowth and maintenance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene and a pseudogene of this gene is located on the long arm of chromosome X. [provided by RefSeq Mar 2012]

Gene Symbol:   NDEL1
Gene Name:   nudE nuclear distribution gene E homolog (A. nidulans)-like 1
Aliases:   DKFZp451M0318, EOPA, MITAP1, NUDEL
RefSeq:   NC_000017.10 NT_010718.16
Ensembl:   ENSG00000166579
Entrez:   81565
Chromosome Mapping:   17p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.710000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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