PrimePCR™ Probe Assay: ADCY4, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057530
Assay Design:   exonic
Chromosome Location:   14:24798272-24798398question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000279027 ENST00000472148 ENST00000413164 ENST00000290317 ENST00000396360 ENST00000435032 ENST00000232744 ENST00000453791 ENST00000468137 ENST00000393363 ENST00000371746 ENST00000371748 ENST00000194900 ENST00000374360 ENST00000374355 ENST00000542398 ENST00000355803 ENST00000325437 ENST00000397514 ENST00000397515 ENST00000310574 ENST00000573128 ENST00000572582 ENST00000574457 ENST00000310677 ENST00000554068 ENST00000418030 ENST00000556932 ENST00000396747 ENST00000397335 ENST00000542125 ENST

Gene Information

This gene encodes a member of the family of adenylate cyclases which are membrane-associated enzymes that catalyze the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). Mouse studies show that adenylate cyclase 4 along with adenylate cyclases 2 and 3 is expressed in olfactory cilia suggesting that several different adenylate cyclases may couple to olfactory receptors and that there may be multiple receptor-mediated mechanisms for the generation of cAMP signals. Alternative splicing results in transcript variants. [provided by RefSeq Nov 2010]

Gene Symbol:   ADCY4
Gene Name:   adenylate cyclase 4
Aliases:   AC4
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000129467
Entrez:   196883
UniGene:   Hs.443428
Chromosome Mapping:   14q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.910000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download