PrimePCR™ Probe Assay: ZNF213, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ZNF213, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF213, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057517
Assay Design:   exonic
Chromosome Location:   16:3191387-3191534question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000579585 ENST00000584573 ENST00000361733 ENST00000361357 ENST00000577395 ENST00000526508 ENST00000524625 ENST00000359513 ENST00000434074 ENST00000312040 ENST00000451945 ENST00000272433 ENST00000369983 ENST00000397928 ENST00000265113 ENST00000574902 ENST00000396878 ENST00000576416 ENST00000572647 ENST00000416391 ENST00000278935 ENST00000306601 ENST00000366471 ENST00000366469 ENST00000451251 ENST00000427146

Gene Information

C2H2 zinc finger proteins such as ZNF213 have bipartite structures in which one domain binds DNA or RNA and the other modulates target gene expression.[supplied by OMIM Apr 2004]

Gene Symbol:   ZNF213
Gene Name:   zinc finger protein 213
Aliases:   CR53, ZKSCAN21
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000085644
Entrez:   7760
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.810000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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