PrimePCR™ Probe Assay: TAS2R43, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TAS2R43, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TAS2R43, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0056152
Assay Design:   exonic
Chromosome Location:   12:11244151-11244297question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000572663 ENST00000574336 ENST00000575161 ENST00000573885 ENST00000571012 ENST00000538986 ENST00000390673 ENST00000390675 ENST00000533467 ENST00000531678 ENST00000539585 ENST00000570941 ENST00000573562 ENST00000572376 ENST00000574263 ENST00000571879 ENST00000576640

Gene Information

TAS2R43 belongs to the large TAS2R receptor family. TAS2Rs are expressed on the surface of taste receptor cells and mediate the perception of bitterness through a G protein-coupled second messenger pathway (Conte et al. 2002 [PubMed 12584440]). For further information on TAS2Rs see MIM 604791.[supplied by OMIM Mar 2009]

Gene Symbol:   TAS2R43
Gene Name:   taste receptor, type 2, member 43
Aliases:   T2R43, T2R52
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000255374
Entrez:   259289
Chromosome Mapping:   12p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.900000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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