PrimePCR™ Probe Assay: NFS1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NFS1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NFS1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0056058
Assay Design:   exonic
Chromosome Location:   20:34285639-34286411question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000371941 ENST00000396220 ENST00000565459 ENST00000374092 ENST00000374085 ENST00000397425 ENST00000419569 ENST00000306750 ENST00000541387 ENST00000540053 ENST00000419816 ENST00000278878 ENST00000378486 ENST00000378488 ENST00000380470 ENST00000344384 ENST00000480796 ENST00000537676 ENST00000334456 ENST00000544570 ENST00000418754 ENST00000540345 ENST00000475807 ENST00000538299 ENST00000444035 ENST00000263934 ENST00000377086 ENST00000377081 ENST00000361099 ENST00000409465 ENST00000392322 ENST

Gene Information

Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described. [provided by RefSeq Nov 2010]

Gene Symbol:   NFS1
Gene Name:   NFS1 nitrogen fixation 1 homolog (S. cerevisiae)
Aliases:   IscS, NIFS
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000244005
Entrez:   9054
Chromosome Mapping:   20q11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.470000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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