PrimePCR™ Probe Assay: HOGA1, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0056038
Assay Design:   exonic
Chromosome Location:   10:99372115-99372216question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000370646 ENST00000370647

Gene Information

The authors of PMID:20797690 cloned this gene while searching for genes in a region of chromosome 10 linked to primary hyperoxalurea type III. They noted that even though the encoded protein has been described as a mitochondrial dihydrodipicolinate synthase-like enzyme it shares little homology with E. coli dihydrodipicolinate synthase (Dhdps) particularly in the putative substrate-binding region. Moreover neither lysine biosynthesis nor sialic acid metabolism for which Dhdps is responsible occurs in vertebrate mitochondria. They propose that this gene encodes mitochondrial 4-hydroxyl-2-oxoglutarate aldolase (EC 4.1.3.16) which catalyzes the final step in the metabolic pathway of hydroxyproline releasing glyoxylate and pyruvate. This gene is predominantly expressed in the liver and kidney and mutations in this gene are found in patients with primary hyperoxalurea type III. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq Nov 2010]

Gene Symbol:   HOGA1
Gene Name:   4-hydroxy-2-oxoglutarate aldolase 1
Aliases:   C10orf65, DHDPS2, DHDPSL, FLJ37472, FLJ56960, HP3, NPL2
RefSeq:   NC_000010.10 NT_030059.13 NG_027922.1
Ensembl:   ENSG00000241935
Entrez:   112817
UniGene:   Hs.180346
Chromosome Mapping:   10q24.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.550000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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