PrimePCR™ Probe Assay: PCDHGC5, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0056027
Assay Design:   exonic
Chromosome Location:   5:140870808-140870906question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000252087

Gene Information

This gene is a member of the protocadherin gamma gene cluster one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes subfamily B contains 7 genes and 2 pseudogenes and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large variable region exons are followed by a constant region containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes. [provided by RefSeq Jul 2008]

Gene Symbol:   PCDHGC5
Gene Name:   protocadherin gamma subfamily C, 5
Aliases:   MGC138286, MGC138288, PCDH-GAMMA-C5
RefSeq:   NC_000005.9 NG_000012.2 NT_029289.11
Ensembl:   ENSG00000240764
Entrez:   56097
UniGene:   Not Available
Chromosome Mapping:   5q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.620000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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