PrimePCR™ Probe Assay: RNF223, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RNF223, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RNF223, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0056009
Assay Design:   exonic
Chromosome Location:   1:1007699-1007796question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000170168 ENST00000409762 ENST00000409582 ENST00000429174 ENST00000413539 ENST00000258104 ENST00000409651 ENST00000394120 ENST00000409744 ENST00000409366 ENST00000410020 ENST00000410041 ENST00000225916 ENST00000405237 ENST00000312108 ENST00000395650 ENST00000360719 ENST00000457250 ENST00000440709 ENST00000434492 ENST00000453464 ENST00000380548 ENST00000321394 ENST00000564173

Gene Information

Description Not Available

Gene Symbol:   RNF223
Gene Name:   ring finger protein 223
Aliases:   Not Available
RefSeq:   NC_000001.10 NT_004350.19
Ensembl:   ENSG00000237330
Entrez:   401934
Chromosome Mapping:   1p36.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.995400
y-intercept 36.670000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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