PrimePCR™ Probe Assay: PGAM4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PGAM4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PGAM4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055957
Assay Design:   exonic
Chromosome Location:   X:77224399-77224548question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000334828 ENST00000432854 ENST00000297283 ENST00000458128 ENST00000441222 ENST00000451218 ENST00000598154

Gene Information

This intronless gene appears to have arisen from a retrotransposition event yet it is thought to be an expressed protein-coding gene. The encoded protein is a member of the phosphoglycerate mutase family a set of enzymes that catalyze the transfer of a phosphate group from 3-phosphoglycerate to 2-phosphoglycerate. [provided by RefSeq May 2010]

Gene Symbol:   PGAM4
Gene Name:   phosphoglycerate mutase family member 4
Aliases:   PGAM-B, PGAM1, PGAM3, dJ1000K24.1
RefSeq:   NC_000023.10 NG_013224.1 NG_021360.1 NT_011651.17
Ensembl:   ENSG00000226784
Entrez:   441531
Chromosome Mapping:   Xq13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.995800
y-intercept 34.420000
Efficiency 109

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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