PrimePCR™ Probe Assay: OVCA2, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055867
Assay Design:   exonic
Chromosome Location:   17:1946594-1946698question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000262441 ENST00000263083 ENST00000571710 ENST00000263084 ENST00000572195 ENST00000413852 ENST00000002829 ENST00000434342 ENST00000359139 ENST00000579146 ENST00000395383 ENST00000361997 ENST00000395386 ENST00000315808 ENST00000395390 ENST00000395382 ENST00000220847 ENST00000280734 ENST00000536215 ENST00000538560 ENST00000463381 ENST00000397238 ENST00000461065

Gene Information

Description Not Available

Gene Symbol:   OVCA2
Gene Name:   ovarian tumor suppressor candidate 2
Aliases:   Not Available
RefSeq:   NC_000017.10 NT_010718.16
Ensembl:   ENSG00000214014
Entrez:   124641
UniGene:   Not Available
Chromosome Mapping:   17p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.070000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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