PrimePCR™ Probe Assay: ARHGAP19, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055843
Assay Design:   exonic
Chromosome Location:   10:99019384-99023323question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000453547 ENST00000316676 ENST00000358308 ENST00000358531 ENST00000371027 ENST00000466484 ENST00000355366

Gene Information

Members of the ARHGAP family such as ARHGAP19 encode negative regulators of Rho GTPases (see RHOA; MIM 165390) which are involved in cell migration proliferation and differentiation actin remodeling and G1 cell cycle progression (Lv et al. 2007 [PubMed 17454002]).[supplied by OMIM Mar 2008]

Gene Symbol:   ARHGAP19
Gene Name:   Rho GTPase activating protein 19
Aliases:   DKFZp313K217, MGC138804, MGC138805, MGC14258
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000213390
Entrez:   84986
UniGene:   Hs.80305
Chromosome Mapping:   10q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.960000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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