PrimePCR™ Probe Assay: RNF208, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055826
Assay Design:   exonic
Chromosome Location:   9:140114936-140115120question
Amplicon Length:   155
Splice Variants Targeted:   ENST00000392827 ENST00000391553 ENST00000311181 ENST00000292928 ENST00000439428 ENST00000435416 ENST00000536254 ENST00000360729 ENST00000588311 ENST00000585696 ENST00000392163 ENST00000409262 ENST00000304710 ENST00000361085 ENST00000351773

Gene Information

Description Not Available

Gene Symbol:   RNF208
Gene Name:   ring finger protein 208
Aliases:   DKFZp761H1710, MGC88636
RefSeq:   NC_000009.11 NT_024000.16
Ensembl:   ENSG00000212864
Entrez:   727800
UniGene:   Hs.512767
Chromosome Mapping:   9q34.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.620000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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