PrimePCR™ Probe Assay: ITSN1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ITSN1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ITSN1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055787
Assay Design:   exonic
Chromosome Location:   21:35093484-35094905question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000399353 ENST00000444491 ENST00000381318 ENST00000381291 ENST00000399367 ENST00000399352 ENST00000399355 ENST00000399349 ENST00000451686 ENST00000381283 ENST00000381285 ENST00000437442 ENST00000399326 ENST00000379960 ENST00000399338

Gene Information

The protein encoded by this gene is a cytoplasmic membrane-associated protein that indirectly coordinates endocytic membrane traffic with the actin assembly machinery. In addition the encoded protein may regulate the formation of clathrin-coated vesicles and could be involved in synaptic vesicle recycling. This protein has been shown to interact with dynamin CDC42 SNAP23 SNAP25 SPIN90 EPS15 EPN1 EPN2 and STN2. Multiple transcript variants encoding different isoforms have been found for this gene but the full-length nature of only two of them have been characterized so far. [provided by RefSeq Jul 2008]

Gene Symbol:   ITSN1
Gene Name:   intersectin 1 (SH3 domain protein)
Aliases:   ITSN, MGC134948, MGC134949, SH3D1A, SH3P17
RefSeq:   NC_000021.8 NT_011512.11
Ensembl:   ENSG00000205726
Entrez:   6453
Chromosome Mapping:   21q22.1-q22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.290000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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