PrimePCR™ Probe Assay: MT1M, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MT1M, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MT1M, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055768
Assay Design:   exonic
Chromosome Location:   16:56667690-56667816question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000389840 ENST00000585328 ENST00000355451 ENST00000262304 ENST00000423118 ENST00000331366 ENST00000538596 ENST00000306317 ENST00000424267 ENST00000517694 ENST00000392668 ENST00000345075 ENST00000535549 ENST00000342754 ENST00000330010 ENST00000256246 ENST00000253506 ENST00000397790 ENST00000427363 ENST00000318065 ENST00000329101 ENST00000545796 ENST00000442561 ENST00000394009 ENST00000553773 ENST00000436008 ENST00000406686 ENST00000380302 ENST00000490273 ENST00000292852 ENST00000374695 ENST

Gene Information

This gene encodes a member of the metallothionein superfamily type 1 family. Metallothioneins have a high content of cysteine residues that bind various heavy metals. These genes are transcriptionally regulated by both heavy metals and glucocorticoids. [provided by RefSeq Oct 2011]

Gene Symbol:   MT1M
Gene Name:   metallothionein 1M
Aliases:   MGC118949, MGC40498, MT-1M, MT-IM, MT1, MT1K
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000205364
Entrez:   4499
Chromosome Mapping:   16q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.510000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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