PrimePCR™ Probe Assay: ZNF616, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ZNF616, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF616, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055718
Assay Design:   exonic
Chromosome Location:   19:52627195-52633758question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000439461 ENST00000601711 ENST00000599581 ENST00000600821 ENST00000595149 ENST00000595000 ENST00000593612 ENST00000359102 ENST00000594295 ENST00000594440 ENST00000389534 ENST00000600228 ENST00000330123 ENST00000596290 ENST00000597013 ENST00000600282 ENST00000597252 ENST00000596428 ENST00000597065 ENST00000322146

Gene Information

Description Not Available

Gene Symbol:   ZNF616
Gene Name:   zinc finger protein 616
Aliases:   MGC149778, MGC45556
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000204611
Entrez:   90317
Chromosome Mapping:   19q13.41

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.580000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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