PrimePCR™ Probe Assay: ZNF468, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ZNF468, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF468, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055717
Assay Design:   exonic
Chromosome Location:   19:53345185-53345321question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000421239 ENST00000601120 ENST00000359798 ENST00000465448 ENST00000486474 ENST00000461321 ENST00000391785 ENST00000301093 ENST00000593941 ENST00000540331 ENST00000602162 ENST00000319783 ENST00000595798 ENST00000601643 ENST00000600943 ENST00000543227 ENST00000540744 ENST00000453741 ENST00000338230 ENST00000438150 ENST00000457749 ENST00000391783 ENST00000596559 ENST00000594602 ENST00000339844 ENST00000360272 ENST00000414252 ENST00000595646 ENST00000243639 ENST00000597924 ENST00000396409 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF468
Gene Name:   zinc finger protein 468
Aliases:   DKFZp781B1474, DKFZp781N07108
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000204604
Entrez:   90333
Chromosome Mapping:   19q13.41

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.750000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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