PrimePCR™ Probe Assay: CASP12, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055712
Assay Design:   exonic
Chromosome Location:   11:104758021-104761188question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000422698 ENST00000375726 ENST00000447913 ENST00000446862 ENST00000441710 ENST00000433738 ENST00000448103 ENST00000494737 ENST00000508062

Gene Information

Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. Several alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq Feb 2011]

Gene Symbol:   CASP12
Gene Name:   caspase 12 (gene/pseudogene)
Aliases:   CASP-12, CASP12P1
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000204403
Entrez:   100506742
UniGene:   Hs.476989
Chromosome Mapping:   11q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.400000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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