PrimePCR™ Probe Assay: CARD16, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055711
Assay Design:   exonic
Chromosome Location:   11:104912424-104915311question
Amplicon Length:   186
Splice Variants Targeted:   ENST00000359866 ENST00000373257 ENST00000332312 ENST00000527979 ENST00000533400 ENST00000436863 ENST00000525825 ENST00000528974 ENST00000598974 ENST00000593315 ENST00000393136 ENST00000375706 ENST00000375704 ENST00000525374 ENST00000528513 ENST00000375707 ENST00000311069 ENST00000373048 ENST00000330210 ENST00000427468 ENST00000540011 ENST00000400677

Gene Information

Description Not Available

Gene Symbol:   CARD16
Gene Name:   caspase recruitment domain family, member 16
Aliases:   COP, COP1, PSEUDO-ICE
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000204397
Entrez:   114769
UniGene:   Hs.348365
Chromosome Mapping:   -

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.230000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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