PrimePCR™ Probe Assay: LRIT2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055694
Assay Design:   exonic
Chromosome Location:   10:85981133-85981282question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000372113 ENST00000538192

Gene Information

Description Not Available

Gene Symbol:   LRIT2
Gene Name:   leucine-rich repeat, immunoglobulin-like and transmembrane domains 2
Aliases:   LRRC22
RefSeq:   NC_000010.10 NG_028034.1 NT_030059.13
Ensembl:   ENSG00000204033
Entrez:   340745
UniGene:   Hs.450425
Chromosome Mapping:   10q23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.800000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download