PrimePCR™ Probe Assay: MAGEE1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MAGEE1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MAGEE1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055664
Assay Design:   exonic
Chromosome Location:   X:75650528-75650640question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000361470 ENST00000597016

Gene Information

This gene encodes an alpha-dystrobrevin-associated MAGE (melanoma-associated antigen) protein which is a member of the MAGE family. The protein contains a nuclear localization signal in the N-terminus 30 12-amino acid repeats beginning at nt 60 with the consensus sequence ASEGPSTSVLPT and two MAGE domains in the C-terminus. It may play a signaling role in brain muscle and peripheral nerve. This gene is located on X chromosome in a region containing loci linked to mental retardation. [provided by RefSeq Mar 2010]

Gene Symbol:   MAGEE1
Gene Name:   melanoma antigen family E, 1
Aliases:   DAMAGE, HCA1, KIAA1587
RefSeq:   NC_000023.10 NG_016774.1 NT_011669.17
Ensembl:   ENSG00000198934
Entrez:   57692
Chromosome Mapping:   Xq13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.110000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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