PrimePCR™ Probe Assay: PRMT6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PRMT6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PRMT6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055655
Assay Design:   exonic
Chromosome Location:   1:107600605-107600743question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000370078 ENST00000361318

Gene Information

The protein encoded by this gene belongs to the arginine N-methyltransferase family which catalyze the sequential transfer of methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. This protein can catalyze both the formation of omega-N monomethylarginine and asymmetrical dimethylarginine with a strong preference for the latter. It specifically mediates the asymmetric dimethylation of Arg2 of histone H3 and the methylated form represents a specific tag for epigenetic transcriptional repression. This protein also forms a complex with and methylates DNA polymerase beta resulting in stimulation of polymerase activity by enhancing DNA binding and processivity. [provided by RefSeq Sep 2011]

Gene Symbol:   PRMT6
Gene Name:   protein arginine methyltransferase 6
Aliases:   FLJ10559, FLJ51477, HRMT1L6
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000198890
Entrez:   55170
Chromosome Mapping:   1p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.170000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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