This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: PRMT6, Human
PrimePCR™ Template for Probe Assay: PRMT6, Human
The protein encoded by this gene belongs to the arginine N-methyltransferase family which catalyze the sequential transfer of methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. This protein can catalyze both the formation of omega-N monomethylarginine and asymmetrical dimethylarginine with a strong preference for the latter. It specifically mediates the asymmetric dimethylation of Arg2 of histone H3 and the methylated form represents a specific tag for epigenetic transcriptional repression. This protein also forms a complex with and methylates DNA polymerase beta resulting in stimulation of polymerase activity by enhancing DNA binding and processivity. [provided by RefSeq Sep 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.