PrimePCR™ Probe Assay: RASSF9, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055626
Assay Design:   exonic
Chromosome Location:   12:86198949-86199051question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000361228

Gene Information

The protein encoded by this gene localizes to perinuclear endosomes. This protein associates with peptidylglycine alpha-amidating monooxygenase and may be involved with the trafficking of this enzyme through secretory or endosomal pathways. [provided by RefSeq Jul 2008]

Gene Symbol:   RASSF9
Gene Name:   Ras association (RalGDS/AF-6) domain family (N-terminal) member 9
Aliases:   P-CIP1, PAMCI
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000198774
Entrez:   9182
UniGene:   Hs.527881
Chromosome Mapping:   12q21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.080000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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