PrimePCR™ Probe Assay: ZNF627, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055586
Assay Design:   exonic
Chromosome Location:   19:11708299-11725344question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000428311 ENST00000339282 ENST00000596193 ENST00000265593 ENST00000344937 ENST00000434054 ENST00000457512 ENST00000423355 ENST00000361113 ENST00000587939 ENST00000588174 ENST00000343916 ENST00000358491 ENST00000597078 ENST00000594605 ENST00000314714 ENST00000424810

Gene Information

Description Not Available

Gene Symbol:   ZNF627
Gene Name:   zinc finger protein 627
Aliases:   FLJ90365
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000198551
Entrez:   199692
UniGene:   Hs.526665
Chromosome Mapping:   19p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.980000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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